Key features and details | |
Cat. No. | MABL-1395 |
Name | Anti-Disialoganglioside 2 mAbs |
Clone No. | AFD- 14G2a |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | therapeutic, ELISA, FC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody binds the GD2 antigen and GD2 expressing neuroblastoma cell lines with an affinity similar to the parent antibody. |
Alternative Name | GD2 |
UniProt | |
Immunogen | The original murine parent antibody was generated against human neuroblastoma cell line LAN-1. This IgG2a isotype version was then generated by class switching of the murine parent antibody 14.18 (IgG3). |
Application Notes | This antibody is a IgG2a isotype switch variant of the murine parent antibody 14.18. This antibody can direct efficient complement-dependent cytotoxicity against neuroblastoma tumor cells and melanoma cell lines in vitro in the presence of human complement (PMID: 2720646). 14G2a was coupled to the ribosome-inactivating plant toxin gelonin with the heterobifunctional cross-linking reagentN- succinimidyl-3(2-pyridyldithio)propionate. The immunoreactivity and specificity of 14G2a-gelonin immunotoxin determined by ELISA. Assays for complement-dependent cytotoxicity (CDC) and antibody- dependent cellular cytotoxicity (ADCC) revealed that these functional properties of the native 14G2a antibody were also preserved in the 14G2a-gelonin immunotoxin (PMID: 1756537). |
Antibody First Published | Mujoo et al. Functional properties and effect on growth suppression of human neuroblastoma tumors by isotype switch variants of monoclonal antiganglioside GD2 antibody 14.18. Cancer Res. (1989); 49(11):2857-61. PMID: 2720646 |
Note on publication | Describes the generation of isotype variants of murine parent antibody 14.18. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
