Key features and details | |
Cat. No. | MABL-1428 |
Name | Anti-DPGA anthrax mAbs |
Clone No. | AFD- F26G3 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | in vivo, neutralize, ELISA, IF |
Species Reactivity | Bacillus anthracis |
Basic Information | |
Specificity | This antibody specifically binds the poly-γ-D-glutamic acid (γDPGA) capsule of Bacillus anthracis. Bacillus anthracis is surrounded by an antiphagocytic polypeptide capsule composed of poly γ-d- glutamic acid (γDPGA). The γDPGA capsule shields the vegetative form of B. anthracis from agglutination by monoclonal antibodies to its cell wall polysaccharide. γDPGA has been identified recently as a potential target for vaccine development. Bacillus anthracis is a spore-forming bacterium and a causative agent for Anthrax, which is a highly lethal infectious disease in human and poses a great threat as an emerging bioterror agent. |
Alternative Name | DPGA; capsular antigen; γDPGA; gamma DPGA; poly-gamma-d-glutamic acid; poly-γ-d-glutamic acid; antiphagocytic polypeptide capsule |
UniProt | |
Immunogen | The original antibody was generated by immunizing BALB/c mice with B. licheniformis γDPGA in combination with CD40 agonist antibody. |
Application Notes | The binding of this antibody to B. anthracis capsule was assessed by direct immunofluorescence using the Alexa Fluor 488 (Molecular Probes) labeled antibody. An antigen capture ELISA was developed, where this antibody was used as capture antibody and a horseradish peroxidase conjugate of this antibody was used as an indicator antibody. The assessment of assay sensitivity was done using γDPGA from B. licheniformis demonstrated that the assay could detect γDPGA at concentrations as low as 100-140 pg/ml. Passive immunization with this antibody protected >90% of mice in a pulmonary model of anthrax that was lethal in control mice (PMID: 15051894). A 50:50 mixture of PDGA antibodies F24F2 and F26G3 was used in the development of an antigen capture ELISA with a sensitivity of approximately 9 ng/ml of serum after a 1/40 dilution and 2.25 ng/ml for a 1:10 dilution (PMID: 19506008). |
Antibody First Published | Kozel et al. MAbs to Bacillus anthracis capsular antigen for immunoprotection in anthrax and detection of antigenemia. Proc Natl Acad Sci U S A. 2004 Apr 6; 101(14): 5042–5047. PMID:15051894 |
Note on publication | Describes the generation of two antibodies against B. anthracis γDPGA and evaluates their capacity to provide protection in pulmonary mouse model of anthrax. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
