Key features and details | |
Cat. No. | MABL-1429 |
Name | Anti-DR4 mAbs |
Clone No. | AFD- HGS-ETR1 (Mapatumumab; TRM-1) |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | agonist, functional assay, in vivo, ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody binds human CD261 (TR4 or TRAILR1 or TNFRSF10A). This protein acts as a receptor for the cytotoxic ligand TNFSF10/TRAIL and transduces cell death signal and induces cell apoptosis. |
Alternative Name | CD261; TR4; TNFRSF10A; APO2; TRAILR1; Tumor necrosis factor receptor superfamily member10A; Death receptor 4; TNF-related apoptosis-inducing ligand receptor 1; T1014A04 |
UniProt | O00220 |
Immunogen | The original antibody was isolated from a human scFv library by panning against human TR4 fusion protein. Later on the scFv version of this antibody was converted to human IgG1 version for carrying out further studies. |
Application Notes | The binding specificity of this antibody to human TR4 (CD126) fusion protein was determined using ELISA. This antibody does not cross react with other proteins like TR7, TR5, TR10 and BLyS. It was reported that the scFv version of the antibody binds human TR4 fusion protein with a binding affinity of Kd= 4.68 × 10^(-10) nM. The ability of this antibody to induce apoptosis of TR4 expressing cells, alone or in combination with chemotherapeutic or cross-linking agents was studied in TR4 expressing cell lines, SW480 and HeLa. The IgG1 format of this antibody induces apoptosis of SW480 cells in the presence of a cross linking agent, but in the absence of cycloheximide. In the presence of cycloheximide, but with or without a crosslinking reagent, the IgG1 antibody induces apoptosis of SW480 and HeLa cells. It was also reported that when administered in vivo with Topotecan, this antibody was capable of retarding the growth of tumor cells in nude mice (US7064189). |
Antibody First Published | Pukac et al. HGS-ETR1, a fully human TRAIL-receptor 1 monoclonal antibody, induces cell death in multiple tumor types in vitro and in vivo. Br J Cancer. 2005 Apr 25;92(8):1430-41. PMID:15846298 |
Note on publication | Describes the generation of an agonistic antibody against human TRAIL receptor 1. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
