Key features and details | |
Cat. No. | MABL-1453 |
Name | Anti-E Cadherin mAbs |
Clone No. | AFD- 56-4 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | agonist, ELISA |
Species Reactivity | Mouse |
Basic Information | |
Specificity | This antibody binds mouse E-cadherin. Cadherins are calcium-dependent cell adhesion proteins. It is localized on the surfaces of epithelial cells in regions of cell-cell contact known as adherens junctions. E-cadherins are reported to play an important role in cancer metastasis. |
Alternative Name | CD324; ARC-1; Cdh1; Cadherin-1; Cadherin E; E-Cadherin; Uvomorulin |
UniProt | P09803 |
Immunogen | The original antibody was generated by immunizing rabbits with purified extracellular domain of mouse E-cadherin. |
Application Notes | The binding of this antibody to mouse E-cadherin results in its activation. The initial binding characterization of this antibody to mouse E-cadherin was done using ELISA. This antibody was used to study the effect of activation of mouse E-cadherin on the growth and metastasis of a spontaneous breast cancer mouse model. Female MMTV-PyMT and control mice treated with 5 mg/kg of this antibody developed tumors in all 10 mammary glands. Also the number of metastasis nodules was significantly reduced with treatment with 56-4 activating antibody (PMID: 32127478; WO2020243616). |
Antibody First Published | Young Na et al. The functional activity of E-cadherin controls tumor cell metastasis at multiple steps. Proc Natl Acad Sci U S A. 2020 Mar 17;117(11):5931-5937. PMID:32127478 |
Note on publication | The paper describes the use of activating antibodies to determine the role of E- cadherin in regulation of metastasis. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
