Key features and details | |
Cat. No. | MABL-1470 |
Name | Anti-EGFR mAbs |
Clone No. | AFD- 7D12 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Blocking, crystallization, FACS, in vivo, inhibition, SPR, ELISA |
Species Reactivity | Monkey, Human |
Basic Information | |
Specificity | The antibody is specific for EGFR. The epitope lies on domain III of the EGFR. The antibody sterically inhibits EGF binding to its receptor. |
Alternative Name | EC:2.7.10.1; EGFR#1; Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1 |
UniProt | P00533 |
Immunogen | The original antibody was generated by immunizing Llama glama with human epidermal A431 carcinoma cells. After cloning variable domains of the heavy-chain antibodies from the peripheral blood and lymph node lymphocytes, a phage-displayed library was constructed. The antibody was isolated by panning on immobilized EGFRs. |
Application Notes | The binding affinity of the VHH fragment to the EGFR extracellular region was measured by surface plasmon resonance. The crystal structure of the antibody in complex with EGFR was determined (Schmitz et al. 2013; PMID: 23791944). The specificity of the antibody to human EGFRvIII protein and monkey EGFR was confirmed by ELISA analysis. The antibody could bind to the human EGFR protein on the surface of A431 cells and CHO-K1-human EGFR 1D4 cells and monkey EGFR protein on the surface of 293 and HEK293T cells by FACS analysis. The antibody bound to human EGFRvIII protein on the surface of CHO-K1-EGFRvIII1C6 cells by FACS analysis. The binding affinity of the Fab and IgG fragments to VEGFR-2 was measured by surface plasmon resonance (Kd= 4 nM) (WO2022121928A1). The 99mTc-labeled antibody could block EGFR on A431 cells in in vitro experiments. Mice bearing subcutaneous A431 (EGFR- positive) and R1M (EGFRnegative) xenografts were intravenously injected with the labeled antibody. |
Antibody First Published | Gainkam et al. Comparison of the biodistribution and tumor targeting of two 99mTc-labeled anti-EGFR nanobodies in mice, using pinhole SPECT/micro-CT J Nucl Med. 2008 May;49(5):788-95. doi: 10.2967/jnumed.107.048538. Epub 2008 Apr 15. PMID:18413403 |
Note on publication | The original paper describes the generation and characterization of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
