Anti-Endothelial Progenitor Cell mAbs

Cat. No.

MABL-1490

Name

Anti-Endothelial Progenitor Cell mAbs

Clone No.

AFD- UG1

From

Recombinant Antibody

Isotype

Engineer antibody

Application

FC

Species Reactivity

Human

Basic Information

Specificity

UG1 binds predominantly to a subset of CD14+   monocytes that mostly co-express lymphocyte markers but UG1 does not preferentially bind to cells which express receptors for proangiogenic   factors.

Alternative Name

gUG1; colony-forming unit Hill cells;   CFU-Hills; circulating angiogenic cells; CACs; endothelial colony-forming   cells; ECFCs; early outgrowth cells; early EPCs; late outgrowth cells; late   EPCs

UniProt

Immunogen

Peripheral blood was obtained from human   donors. The PBMCs were obtained by density centrifugation and cultured. Two   ISA brown chickens were then injected intradermally and after 50 days the   spleen and bone marrow was harvested. RT-PCR was performed on cells from the   spleen and bone marrow which was used to generate a cDNA library. Primers   were then used to amplify the VL and VH genes and this was assembled in an   scFv format. Phage libraries were generated and the library was biopanned.   The biopanned library was then transformed into XL1 blue bacteria and   colonies chosed at random from these.

Application Notes

Has been used in flow cytometry to show the   population of cells that UG1 binds to. This was shown to be almost   exclusively CD14+ and CD34- and shows no preference for cells expressing   Tie-2 and CD309 which recognise angiogenic peptides. There was also a higher   proportion of CD56, CD3, and CD19 (Bowes et al).

Antibody First   Published

Bowes et al Developing cell-specific   antibodies to endothelial progenitor cells using avian immune phage display   technology. J Biomol Screen. 2011 Aug;16(7):744-54 PMID:21593485

Note on publication

Describes the generation of a library of   phage displays and scFvs against a heterogeneous population of peripheral   blood mononuclear cells (PBMC). It goes on to discuss the characterisation of   three of these antibodies and the codon optimisation of UG1.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.