Key features and details | |
Cat. No. | MABL-1491 |
Name | Anti-Enrofloxacin mAbs |
Clone No. | AFD- A2 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA |
Species Reactivity | Species independent |
Basic Information | |
Specificity | This antibody is a specific binder for enrofloxacin and did not cross-react with other fluoroquinolone antibiotics including structurally similar ciprofloxacin. |
Alternative Name | Baytril |
UniProt | |
Immunogen | The original antibody was isolated from a human scFv phage display library by panning against biotinylated enrofloxacin antibiotic. |
Application Notes | This antibody was capable of binding the biotin analogue of enrofloxacin in an ELISA. The binding affinity of the original scFv antibody to enrofloxacin-biotin analogue was reported to be Kd = 2.5 µM. This antibody was used in the development of an ScFv biosensor for qualitative detection of antibiotics in livestock drinking water (PMID: 21142055), |
Antibody First Published | Young Cha et al. Pharmacophore-based strategy for the development of general and specific scFv biosensors for abused antibiotics. Bioconjug Chem. 2011 Jan 19;22(1):88-94. PMID:21142055 |
Note on publication | Describes the development of a fluorescent biosensor system using antibodies for detection of fluoroquinolone antibiotics |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
