Key features and details | |
Cat. No. | MABL-1504 |
Name | Anti-EpCAM mAbs |
Clone No. | AFD- MOC31 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IHC;, SPR, ELISA, FC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for human EpCAM. |
Alternative Name | CD326; Ep-CAM; KS1/4 antigen; KSA; Epithelial cell adhesion molecule; Adenocarcinoma-associated antigen; Cell surface glycoprotein Trop-1; Epithelial cell surface antigen; Epithelial glycoprotein; EGP; Epithelial glycoprotein 314; 17-1A-antigen; EGP314; hEGP314; Major gastrointestinal tumor-associated protein GA733-2; Tumor-associated calcium signal transducer 1; EGP-2; EGP40; KSA; CO17-1A; GA733-2; MOC-31 |
UniProt | P16422 |
Immunogen | The original antibody was generated by immunizing mice with human EGP-2 antigen. |
Application Notes | The binding affinity of this antibody for human EpCAM was determined using surface plasmon resonance and ELISA. The scFv version of this antibody was used to immunohistochemically stain human carcinoma cells, colonic epithelium, colon carcinoma and melanoma (PMID: 9836471). This antibody binds EpCAM-positive Kato III cell line in a flow cytometric assay. The IgG2a format of this antibody was used for the immunohistochemical analysis of normal human tissues and cancerous tissues. In immunohistochemical studies, MOC31 showed binding towards colon cancers, breast cancer and lung cancer with epithelial origin (PMID: 24525727). This antibody is also reported to be used in differentiation between adenocarcinoma and reactive mesothelial cells (PMID: 10603193). This antibody was also used for immunohistochemical staining of adenocarcinomas (PMID: 16329115). |
Antibody First Published | Roovers et al. High-affinity recombinant phage antibodies to the pan- carcinoma marker epithelial glycoprotein-2 for tumour targeting. Br J Cancer. 1998 Dec;78(11):1407-16. PMID:9836471 |
Note on publication | Describes the generation of high affinity recombinant phage antibodies. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
