Anti-EpCAM mAbs

Cat. No.

MABL-1505

Name

Anti-EpCAM mAbs

Clone No.

AFD- MT201   (Adecatumumab; HD69)

From

Recombinant Antibody

Isotype

Engineer antibody

Application

therapeutic, WB, ELISA, FC, IHC

Species Reactivity

Human

Basic Information

Specificity

This antibody is specific for human EpCAM.

Alternative Name

CD326; Ep-CAM; KS1/4 antigen; KSA;   Epithelial cell adhesion molecule; Adenocarcinoma-associated antigen; Cell   surface glycoprotein Trop-1; Epithelial cell surface antigen; Epithelial   glycoprotein; EGP; Epithelial glycoprotein 314; 17-1A-antigen; EGP314;   hEGP314; Major gastrointestinal tumor-associated protein GA733-2;   Tumor-associated calcium signal transducer 1; EGP-2; EGP40; KSA; CO17-1A;   GA733-2

UniProt

P16422

Immunogen

The original antibody was generated by   panning against a naturally expressed human library.

Application Notes

Immunohistochemistry was performed on human   metastatic breast cancer tissue using this antibody (Schmidt et al, 2010;  pmid:19633042). ELISA was performed on recombinant EpCAM protein using this   antibody (Raum et al, 2000; pmid:11419181). Flow cytometry using this antibody was done on cells (Raum et al, 2000; pmid:11419181). Western blot   was performed on 119 polypeptides of 13 amino acids shifted by two amino acids covering the entire extracellular domain of EpCAM. This antibody was used (Raum et al, 2000; pmid:11419181). Surface plasmon resonance was   performed on the EpCAM protein using this antibody (Raum et al, 2000;   pmid:11419181). This antibody was used for flow cytometery on KATO III cells (Naundorf et al, 2002; pmid:12115595). A cytotoxicity experiment was   performed on PMBC using this antibody (Naundorf et al, 2002; pmid:12115595).   Phase II trials were performed on prostate cancer patients (Marschner et al,   2010; pmid:20606402). Phase I trials were performed on prostate cancer   patients (Oberneder et al, 2006; pmid:16930989).

Antibody First   Published

Raum et al. Anti-self   antibodies selected from a human IgD heavy chain repertoire: a novel approach   to generate therapeutic human antibodies against tumor-associated   differentiation antigens. Cancer Immunol Immunother. 2001 May;50(3):141-50.   PMID:11419181

Note on publication

Human antibodies were   isolated by phage display from a naturally expressed human antibody   repertoire.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.