Anti-FedF mAbs

Cat. No.

MABL-1571

Name

Anti-FedF mAbs

Clone No.

AFD- NbFedF9

From

Recombinant Antibody

Isotype

Engineer antibody

Application

Blocking, MST, SPR, ELISA

Species Reactivity

E. coli

Basic Information

Specificity

Specific to the N terminal lectin domain as   this is the domain represented in the 15-165 fragment. It has an affinity for   FedF(15–165) of around 1.5 nM (Moones et al, 2014).

Alternative Name

F18 fimbrial adhesin AC; F18 fimbrial   adhesin

UniProt

Q47212

Immunogen

A Llama was immunised 6 times with purified FedF   residues 15-165 over a period of 6 weeks. Lymphocytes were extracted and used   to prepare cDNA which was used as a template fthe VHH. These were then   transfected into E. coli and the antibodies selected by phage display. This   was then subjected to ELISA to confirm the specificity of the antibodies.

Application Notes

It has been shown that this antibody can   inhibit the binding of FedF to piglent enterocytes. The structure of this   antibodybinding to FedF has been resolved to a resolution of 1.5A. This shows that the mechanism of inhibition of FedF binding to epithelial cell   surfaces is by competing at the carbohydrate binding site. Both MST and SPR   have been used to analyse binding affinities (Moones et al, 2014).

Antibody First   Published

Moonens et al Nanobody Mediated Inhibition of Attachment of F18 Fimbriae Expressing Escherichia coli PLoS One. 2014; 9(12): e114691. PMID: 25502211

Note on publication

Discusses the generation of this antibody,   its crystallisation and the method of netralisation of FedF

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.