Key features and details | |
Cat. No. | MABL-1595 |
Name | Anti-Fibronectin mAbs |
Clone No. | AFD- C6 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | in vivo, SPR, WB, ELISA, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for a cryptic epitope of human fibronectin. The epitope is localized on the fibronectin type III repeat 8, immediately downstream of the angiogenesis-associated extra-domain B, unmasked by its insertion. The specificity of this antibody for the B-FN isoform is due to the E-F loop of the ED-B, whereas the human specificity is due to the B-C loop of III8. In the majority of other mammalian species, there is an E residue instead of D1385, which is sufficient to abolish the interaction. |
Alternative Name | FN; Cold-insoluble globulin; CIG; EDB; ED-B; B-FN; Extra-domain B fibronectin |
UniProt | P02751 |
Immunogen | The original antibody was generated by immunizing BALB/c mice with recombinant FN fragment composed of the type III repeats 7.EDB.8.9. |
Application Notes | The original version of this antibody (mouse IgG1) was generated and used in ELISA, WB, IHC, SPR, and in vivo assays. ELISA analysis showed that the antibody reacts with type-III-repeats- containing FN fragments "7.EDB.8.9" and "7.EDB", and a Kd of 10 nM was measured in an SPR assay with 7.EDB.8.9 as the antigen. Western blot analysis showed that the epitope recognized by this antibody is located within the type III repeat 8 of FN. IHC analysis showed that this antibody strongly binds human- specific epitopes on repeat III8 of FN in neoplastic tissues. A murine urokinase-type plasminogen activator- conjugated mini-antibody version of the antibody was generated and utilized for in vivo tumor-targeting experiments in tumor-bearing mice expressing human B-FN using a radioiodinated mini-antibody. The distribution of the radioiodinated mini-antibody was assessed in tumor tissue and blood at different time points after injection, and the results showed rapid clearance from the bloodstream. |
Antibody First Published | Balza et al. A novel human fibronectin cryptic sequence unmasked by the insertion of the angiogenesis-associated extra type III domain B. Int J Cancer. 2009 Aug 15;125(4):751-8. PMID:19479996 |
Note on publication | The original publication unveils a novel cryptic sequence within human fibronectin, unmasked by the insertion of the angiogenesis-associated extra type III domain B, with implications for tumor targeting and angiogenesis research. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
