Key features and details | |
Cat. No. | MABL-1594 |
Name | Anti-Fibronectin mAbs |
Clone No. | AFD- BC-1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | RIA, WB, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is selective for a cryptic epitope in domain 7 of the B-form splice variant ED-B sequence of fibronectin (EDB-FN). This antibody does not react with plasma fibronectin or thermolysin- digested fibronectin from Wi-38 or Wi-38Val3 cells. Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. They are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. |
Alternative Name | FN; EDB(+) FN; ED-B domain of fibronectin; Extra domain-B containing fibronectin; oncofetal fibronectin; cold-insoluble globulin; CIG; BC1; HuBC-1 |
UniProt | P02751 |
Immunogen | The original mouse antibody was generated by immunizing mice with fibronectin derived from a culture medium of SV40-transformed Wi-38 embryonic human lung cells and defined Wi-38Val3. The humanized version was generated later by grafting CDRs of original mouse antibody onto human framework regions. |
Application Notes | This antibody is the recombinant humanized version of original anti-fibronectin mouse antibody BC-1. The binding specificity of this antibody for fibronectins was determined using western blotting and radioimmunoassay. This antibody showed strong reaction with WI-38VA13 FN fragments, negative reaction with plasma FN fragments, and a barely detectable reaction with WI-38 FN fragments in a western blot. This antibody was used in the immunohistochemical analysis of the distribution of the ED-B- containing fibronectin isoform (B-FN) in human tissues. The results obtained indicated that fibronectin has a widespread distribution in normal and adult human tissues but the B-FN isoform is restricted only to synovial cells, to some vessels and areas of the interstitium of the ovary, and to the myometrium. Additionally, the B- FN isoform is restricted only to synovial cells, to some vessels and areas of the interstitium of the ovary, and to the myometrium (PMID: 2646306). |
Antibody First Published | Carnemolla et al. A tumor-associated fibronectin isoform generated by alternative splicing of messenger RNA precursors. J Cell Biol. 1989 Mar;108(3):1139-48. PMID:2646306 |
Note on publication | Describes the production and characterization of an antibody specific for the ED-B domain of fibronectin. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
