Anti-FLT3 mAbs

Cat. No.

MABL-1615

Name

Anti-FLT3 mAbs

Clone No.

AFD- EB10

From

Recombinant Antibody

Isotype

Engineer antibody

Application

SPR, ELISA, FC

Species Reactivity

Human

Basic Information

Specificity

The antibody binds an epitope within domain D4 of human FLT3.

Alternative Name

FLK-2; FLT-3; STK-1; CD135; Fetal liver   kinase 2; FL cytokine receptor; stem cell tyosine kinase 1; Fms-like tyrosine   kinase 3; Receptor-type tyrosine-protein kinase FLT3

UniProt

P36888

Immunogen

The original Fab antibody was isolated from a human Fab phage display by selection of a high affinity antibody binding to the human FLT3.

Application Notes

The clone was shown to selectively bind to   human FLT3 with high affinity, block FL binding and mediated potent   anti-tumor activity in xenograft model by a mechanism involving activation of   immune effector function. The ability of the antibody to bind the receptor   was examined by ELISA (EC50 within the range 0.5-1 nM). The ability of the   antibody to block receptor-ligand binding was examined in a FL binding   competition ELISA, the antibody blocked the binding of FL to FLT3 with a IC50   within the range of 0.5-1 nM. The clone binds FLT3 with an affinity of 0.158   nM as determined by surface plasmon resonance (SPR). Flow cytometric analysis   showed that the antibody bound to the wild-type FLT-3 expressed on EOL-1   cells and also to ITD-mutant FLT3 expressed on BaF3-ITD cells (US8071099B2).

Antibody First   Published

Li et al. Anti-FLT3 antibodies

Note on publication

The patent describes a fully human antibody   which specifically bind to human FLT3 within extracellular domains 4 or 5   with high affinity

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.