Anti-FLT3 mAbs

Cat. No.

MABL-1616

Name

Anti-FLT3 mAbs

Clone No.

AFD- NC7

From

Recombinant Antibody

Isotype

Engineer antibody

Application

SPR, ELISA, FC

Species Reactivity

Human

Basic Information

Specificity

The antibody binds an epitope within domain   D5 of human FLT3.

Alternative Name

FLK-2; FLT-3; STK-1; CD135; Fetal liver   kinase 2; FL cytokine receptor; stem cell tyosine kinase 1; Fms-like tyrosine   kinase 3; Receptor-type tyrosine-protein kinase FLT3

UniProt

P36888

Immunogen

The original Fab antibody was isolated from a human Fab phage display by selection of a high affinity antibody binding to the human FLT3.

Application Notes

The clone was shown to selectively bind to   human FLT3 with high affinity and block FL binding. The ability of the   antibody to bind the receptor was examined by ELISA (EC50= 0.3 nM). The   ability of the antibody to block receptor-ligand binding was examined in a FL binding competition ELISA, the antibody blocked the binding of FL to FLT3   with a IC50 within the range of 0.8 nM. The clone binds FLT3 with an affinity of 0.45 nM as determined by surface plasmon resonance (SPR). The in vivo efficacy of the clone was determined in SEM-K2 cells, a human ALL cell line   expressing wild type FLT3. In comparison to human IgG, the clone treatment all significantly prolonged survival in the SEM-K2 leukemia model (US8071099B2).

Antibody First   Published

Li et al. Anti-FLT3 antibodies

Note on publication

The patent describes a fully human antibody   which specifically bind to human FLT3 within extracellular domains 4 or 5   with high affinity

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.