Anti-FSH receptor mAbs

Cat. No.

MABL-1627

Name

Anti-FSH receptor mAbs

Clone No.

AFD- 9H11

From

Recombinant Antibody

Isotype

Engineer antibody

Application

therapeutic, ELISA, FC, IF

Species Reactivity

Human, Mouse

Basic Information

Specificity

This antibody binds human and mice FSH   receptor. FSH receptor is a G protein-coupled receptor for follitropin, the   follicle-stimulating hormone. Through cAMP production activates the   downstream PI3K-AKT and ERK1/ERK2 signaling pathways.

Alternative Name

FSHR; FSH-R; Follicle-stimulating hormone   receptor; Follitropin receptor; LGR1

UniProt

P23945

Immunogen

The original antibody was generated by immunizing Balb/c mice with an optimized DNA construct encoding human FS R

Application Notes

To test if the antibody dependent cellular   cytotoxicity elicited by the mouse version of this antibody translated into   an in vivo anti-tumor effect, mice were challenged with IDS   -Defl29/Vegf-a-Fshr and treated with the mouse version of this antibody. The   antibody was able to delay tumor progression. To further characterize the   antibody its ability to bind cells expressing human and mouse FHSR, flow cytometry was performed using the mouse version of this antibody on A20   cells, ID8- Defl29/Vegf-a-Fshr cells and human ovarian cancer cell lines like   Caov3, OVCAR3, TOV21 G and PC3 with physiological levels of FSHR. Isotype analysis of the antibody was done using ELISA. Finally immunofluorescence was   performed on 293T cells transduced with human or murine FSHR using the mouse   version of this antibody (WO2020033797).

Antibody First   Published

Perales-Puchalt et al. WO2020033797

Note on publication

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.