Key features and details | |
Cat. No. | MABL-1628 |
Name | Anti-Ft OAg mAbs |
Clone No. | AFD- Ab52 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA |
Species Reactivity | Francisella tularensis, Francisella tularensis subsp. Holarctica |
Basic Information | |
Specificity | This antibody strongly binds the internal O-antigen (OAg) of the gram-negative bacterium Francisella tularensis (Ft). |
Alternative Name | O-antigen of Francisella tularensis; O-antigen ligase family protein |
UniProt | A0A0B6E216 |
Immunogen | The original antibody was generated by repeatedly immunizing BALB/c mice with live vaccine strain (LVS) and F. tularensis lipopolysaccharide admixed with CpG ODN 1826 (TCC ATG ACG TTC CTG ACG TT) (an oligodeoxynucleotide containing unmethylated CpG dinucleotides with fully phosphorothioate backbone. |
Application Notes | This potent binder can be used to detect the O antigen of F. tularensis. The antigen specificity of this antibody was tested using ELISA. It is capable of binding to heat killed live vaccine strain and SchuS4 F. tularensis strains in an ELISA. This antibody was also capable of detecting the longer chains of the lipopolysaccharide ladder in a western blot assay (PMID： 21466282). Ab52 can be used as a reporter to monitor the response of vaccine recipients to protective B-cell epitopes of F. tularensis OAg (PMID： 22486311). |
Antibody First Published | Roche et al. Characterization of monoclonal antibodies to terminal and internal o-antigen epitopes of Francisella tularensis lipopolysaccharide. Hybridoma (Larchmt). (2011); 30(1)：19–28. PMID：21466282 |
Note on publication | Describes the generation of antibodies towards terminal and internal O-antigen epitopes of Francisella tularensis lipopolysaccharide. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
