Anti-gamma-delta TCR mAbs

Cat. No.

MABL-1651

Name

Anti-gamma-delta TCR  mAbs

Clone No.

AFD- UC7-13D5

From

Recombinant Antibody

Isotype

Engineer antibody

Application

functional assay, FC

Species Reactivity

Mouse

Basic Information

Specificity

This antibody is specific for mouse gamma   and delta TCR. TCR part of the TCR-CD3 complex present on T-lymphocyte cell   surface that plays an essential role in adaptive immune response. When   antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated   signals are transmitted across the cell membrane by the CD3 chains CD3D,   CD3E, CD3G and CD3Z.

Alternative Name

T-cell surface glycoprotein CD3 delta chain; T-cell receptor T3 delta chain; CD3d; T-cell surface glycoprotein CD3 gamma chain; T-cell receptor T3 gamma chain; CD3g

UniProt

P11942

Immunogen

This antibody was generated by immunizing a   armenian hamster with murine murine gamma-delta TCR.

Application Notes

Alloreactive CD3'CD4- CD8- TCR y/6+ Tcell   lines were used for a cytolytic assay. During the assay this antibody was   used (Rellahan et al, 1991; pmid:1703211). Flow cytometry was preformed on   the spleen cells of mice using this antibody (Koenecke et al, 2009;   pmid:19130484). Flow cytometry was preformed on lymphoid cells of arthritic   mice using this antibody (Peterman et al, 1993; pmid:8245484). A   proliferation assay was preformed on inguinal lymph nodes from arthritic   mice. The mice were treated with this antibody (Peterman et al, 1993;   pmid:8245484).

Antibody First   Published

Rellahan et al. Junctional Sequences   Influence the Specificity of -y/b T Cell Receptors J Exp Med. 1991 Feb   1;173(2):503-6. PMID:1703211

Note on publication

To examine the role of V(D)J junctional sequences in antigen recognition by TCR- gamma/delta, an alloreactive murine   TCR-gamma/delta+ T cell line, LKD1, specific for the I-Ad class II major histocompatibility complex (MHC) molecule, and compared its receptor with   that expressed by a previously characterized class II MHC alloreactive T cell   line, LBK5, specific for I-Ek,b,s Ia molecules was derived.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.