Key features and details | |
Cat. No. | MABL-1650 |
Name | Anti-Gamma Delta TCR mAbs |
Clone No. | AFD- G5-4 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | functional assay, FC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for the human TCR delta and gamma chains. The T-cell receptor (TCR) is a molecule found on the surface of T cells, or T lymphocytes, that is responsible for recognizing fragments of antigen as peptides bound to major histocompatibility complex (MHC) molecules. On activation through antigen presenting cells (APCs), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. TCR part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. |
Alternative Name | TCR γδ; γδ TCR; TCR GD; GD TCR; TCRg/d; T-cell surface glycoprotein CD3 delta chain; CD3D; T3D; T-cell receptor T3 delta chain; T-cell surface glycoprotein CD3 gamma chain; CD3G; T-cell receptor T3 gamma chain; CD3g; T3G |
UniProt | P04234; P09693 |
Immunogen | The original antibody was generated by immunizing BALB/c mice with recombinant γ9δ2 (OT3)-Fc fusion protein as an immunogen. |
Application Notes | The binding of this antibody to γδ T cells was determined using flow cytometry. This antibody can compete with commercially available anti-γδ TCR antibody IMMU510 for binding to gamma- delta T cells. It is proposed that, since there is some competition between the two antibodies, G5-4 binds a similar or identical epitope to IMMU510. In an in vitro proliferation assay, this antibody was reported to positively stimulate the proliferation of γδT cells. The scFv version of this antibody was also reported to stimulate the secretion of cytokines like IFN-γ and TGF-α. G5-4ScFv-amplified γδT cells can effectively kill daudi cells, and the killing efficiency is greater than 85% when the effect-to-target ratio is 5:1 (CN103130894). |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
