Key features and details | |
Cat. No. | MABL-1649 |
Name | Anti-Gamma Delta TCR mAbs |
Clone No. | AFD- B1.1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | functional assay, Block, FC, IF, IHC |
Species Reactivity | Human, Rhesus Monkey, Cynomolgus Monkey |
Basic Information | |
Specificity | This antibody is specific for the human TCR delta and gamma chains. The T-cell receptor (TCR) is a molecule found on the surface of T cells, or T lymphocytes, that is responsible for recognizing fragments of antigen as peptides bound to major histocompatibility complex (MHC) molecules. On activation through antigen presenting cells (APCs), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. TCR part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. |
Alternative Name | TCR γδ; γδ TCR; TCR GD; GD TCR; TCRg/d; T-cell surface glycoprotein CD3 delta chain; CD3D; T3D; T-cell receptor T3 delta chain; T-cell surface glycoprotein CD3 gamma chain; CD3G; T-cell receptor T3 gamma chain; CD3g; T3G; B1 |
UniProt | P04234; P09693 |
Immunogen | Antigen-specific T cell blasts to poly-18, a polypeptide antigen of defined sequence and conformation, were generated from lymph nodes of antigen primed BALB/cCr mice. These blasts were fused with the BW5147 thymoma to obtain anti-poly-18-reactive T cell hybridomas from which the original antibody was generated. |
Application Notes | This antibody can be used for the flow cytometric analysis of human peripheral blood cells (PMID: 12121897; 23447017; 25777993; 28223321). This antibody was used to block human γδ T cell receptor and prevent the human anti-porcine γδ T cell response (PMID: 11502983; 28713391). This antibody was also used to block the γδ T cell receptors in in-vitro tumor-killing assays (PMID: 21633088). This antibody was used for the immunohistochemical analysis of frozen tissue samples of parapsoriasis, mycosis fungoides and inflammatory dermatoses (PMID: 21479436). This antibody was also used for the immunohistochemical and indirect immunofluorescence staining of γδ T cells in breast cancer and normal tissues (PMID: 23959855). |
Antibody First Published | Fotedar et al. Fine specificity of antigen recognition by T cell hybridoma clones specific for poly-18: a synthetic polypeptide antigen of defined sequence and conformation. J Immunol. 1985 Nov;135(5):3028-33. PMID:2413110 |
Note on publication | Describes the epitope characterization of T cell hybridomas specific for Poly-18. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
