Key features and details | |
Cat. No. | MABL-1655 |
Name | Anti-gangliosides mAbs |
Clone No. | AFD- AKH11 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | functional assays, ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for gangliosides GM3 (N-acetylneuraminic acid), GT1b, GD3, GD1a, and GQ1b but does not react with other glycolipids such as lactosylceramide, GM3 (N-glycolylneuraminic acid), GM2, GM1, GD2, GD1b, 9-o-Ac-GD3, SSEA-1, or sialylparagloboside. |
Alternative Name | GM3 (N-acetylneuraminic acid); GT1b; GD3; GD1a; GQ1b |
UniProt | |
Immunogen | The original antibody was generated by preparing and establishing an EBV-transformed human cell line from lymphocytes obtained from a melanoma patient. |
Application Notes | The specificity of the original antibody (human IgM) was assessed using ELISA, revealing that it reacted with gangliosides in the following order: GM3 (N-acetylneuraminic acid) > GT1b > GD3 > GD1a > GQ1b. A recombinant version of the antibody was generated, and its biological activity was evaluated. CDC activity tests demonstrated efficacy against MeWo, M12, M14, and G-361 melanoma cells, while showing no significant CDC activity against human normal skin fibroblasts, human adenocarcinoma, human neuroblastoma, or Vero cells. These findings confirm that the recombinant antibody retains the biological activities of the original AKH11 antibody (EP0492409A1). |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
