Anti-Ganoderic acid A mAbs

Cat. No.

MABL-1656

Name

Anti-Ganoderic acid A mAbs

Clone No.

AFD- 12 A

From

Recombinant Antibody

Isotype

Engineer antibody

Application

ELISA

Species Reactivity

Ganoderma

Basic Information

Specificity

mAb 12 A was shown to react with ganoderic acid A using indirect and competitive ELISAs (Sakamoto et al, 2016). It was also shown that mAb 12 A did not cross react significantly with 32 other   types of Ganoderma triterpenoids. Ganoderic acid A is one of the main   bioactive triterpenoids in the Ganoderma genus of medicinal mushrooms,   traditionally used in medicine.

Alternative Name

GAA; 12A; 12-A

UniProt

Immunogen

BALB/c mice were immunised by injection of ganoderic   acid A-BSA conjugates. Splenocytes were obtained from immunised mice and   fused with SP2/0 murine myeloma cells to generate hybridomas.

Application Notes

mAb 12 has been used in both indirect and   competitive ELISAs, using 33 different types of Ganoderma triterpenoid   including ganoderic acid A (Sakamoto et al, 2016). More recently, mAb 12 has   been reformatted as a scFv fragment and applied in ELISAs for detection of   ganoderic acid A (Sakamoto et al, unpublished).

Antibody First   Published

Sakamoto et al. Detection of Ganoderic Acid   A in Ganoderma lingzhi by an Indirect Competitive Enzyme-Linked Immunosorbent   Assay. Planta Med. 2016 May;82(8):747-51. PMID:27093250

Note on publication

Describes the generation of the   anti-ganoderic acid A mAb 12 A, and preliminary characterisation of its   reactivity using Ganoderma triterpenoids by ELISAs.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.