Key features and details | |
Cat. No. | MABL-1657 |
Name | Anti-GAPDH mAbs |
Clone No. | AFD- lesion-scFv |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for human GAPDH. |
Alternative Name | GAPD; Glyceraldehyde-3-phosphate dehydrogenase; Peptidyl-cysteine S- nitrosylase GAPDH; EC:1.2.1.12; EC:2.6.99. |
UniProt | P04406 |
Immunogen | The original antibody was generated by assembling immunoglobulin VH and VL genes from IgA-positive B cells located in lesions of an MS brain. |
Application Notes | This antibody's original format (human scFv) was used in an ELISA and WB assay (Kolln et al., 2006; PMID: 17015754). |
Antibody First Published | Kolln et al. Triosephosphate isomerase- and glyceraldehyde-3-phosphate dehydrogenase-reactive autoantibodies in the cerebrospinal fluid of patients with multiple sclerosis. J Immunol. 2006 Oct 15;177(8):5652-8. doi: 10.4049/jimmunol.177.8.5652. PMID:17015754 |
Note on publication | The original publication investigates the presence of autoantibodies, including single-chain variable fragment antibodies (scFvs) generated from clonal B cells in the cerebrospinal fluid and lesions of multiple sclerosis (MS) patients, targeting the glycolytic enzymes triosephosphate isomerase (TPI) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH). |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
