Key features and details | |
Cat. No. | MABL-1681 |
Name | Anti-GFP mAbs |
Clone No. | AFD- Clone 1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | FC |
Species Reactivity | Aequorea victoria (Water jellyfish, Mesonema victoria) |
Basic Information | |
Specificity | This antibody binds green fluorescent protein. |
Alternative Name | Green fluorescent protein |
UniProt | P42212 |
Immunogen | The original antibody was isolated from a nanobody library derived from a llama immunized with green fluorescent protein using yeast surface display platform. |
Application Notes | The binding characterization of this antibody for green fluorescent protein was done using flow cytometry. The binding affinity of this antibody for GFP was calculated to be Kd= 2.6nM (PMID： 19861136). |
Antibody First Published | Ryckaert et al. Isolation of antigen-binding camelid heavy chain antibody fragments (nanobodies) from an immune library displayed on the surface of Pichia pastoris. J Biotechnol. 2010 Jan 15;145(2)：93-8. PMID：19861136 |
Note on publication | Describes the use of a yeast surface display platform for rapid discovery of conformationally selective nanobodies. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
