Anti-GFP mAbs

Cat. No.

MABL-1682

Name

Anti-GFP mAbs

Clone No.

AFD- GBP1 (Enh)

From

Recombinant Antibody

Isotype

Engineer antibody

Application

 in vitro, in vivo, IF

Species Reactivity

Species independent

Basic Information

Specificity

This antibody is specific for GFP.

Alternative Name

Green fluorescent protein; GFP Binding   Protein 1; Enhancer

UniProt

P42212

Immunogen

The original antibody was generated by immunizing an alpaca with GFP.

Application Notes

The Enhancer (Enh) anti-GFP nanobody, also   known as GFP binding protein 1 (GBP1), was specifically used for manipulating protein conformation in vitro and in vivo; this nanobody caused a fourfold   fluorescence enhancement by stabilizing the deprotonated, negatively charged   state of the GFP chromophore, resulting in increased fluorescence intensity   (Kirchhofer et al., 2010; PMID： 20010839).

Antibody First   Published

Kirchhofer et al. Modulation of protein   properties in living cells using nanobodies Nat Struct Mol Biol. 2010   Jan;17(1)：133-8. doi: 10.1038/nsmb.1727. Epub 2009 Dec 13 PMID：20010839

Note on publication

The original publication explores the use of   camelid-derived nanobodies to modulate the conformation and spectral   properties of green fluorescent protein (GFP) in living cells, revealing the   potential for manipulating and studying protein conformations using   nanobodies.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.