Key features and details | |
Cat. No. | MABL-1683 |
Name | Anti-GFP mAbs |
Clone No. | AFD- Minimizer |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | crystallization, in vitro |
Species Reactivity | Aequorea victoria (Water jellyfish, Mesonema victoria) |
Basic Information | |
Specificity | The antibody is specific for green fluorescent protein. The antibody interacts with β-strands 6 and 7 of GFP. |
Alternative Name | Green fluorescent protein; MI; GBP4 |
UniProt | P42212 |
Immunogen | The original antibody was generated by immunizing camels with GFP. A phage display library was constructed and the antibody was selected by panning against GFP. |
Application Notes | The antibody could reversibly reduce GFP fluorescence by a factor of 5 in vitro. A comparable fluorescence modulation was also observed after addition of the antibody to soluble cell extract derived from human embryonic kidney (HEK) 293T cells expressing eGFP. The crystal structure of the GFP–antibody complex was determined. The dissociation constant of the antibody was measured (Kd= 0.45 nM) (Kirchhofer et al., 2010; PMID： 20010839). Fluorescence modulation in living cells was also observed after the addition of the antibody to HeLa cells expressing GFP (Schmidthals et al., 2010; PMID： 20372881). |
Antibody First Published | Kirchhofer et al. Modulation of protein properties in living cells using nanobodies. (2010) Nat Struct Mol Biol 17： 133-138 PMID：20010839 |
Note on publication | The original paper describes the generation and characterization of the antibody |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
