Key features and details | |
Cat. No. | MABL-1695 |
Name | Anti-Gintexin A type PLA2 mAbs |
Clone No. | AFD- N20 (3C12) |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | neutralize, WB, ELISA |
Species Reactivity | Gloydius intermedius (Central Asian pit viper, Agkistrodon intermedius) |
Basic Information | |
Specificity | This antibody binds the gintexin A type PLA2 protein found in the venom of Gloydius intermedius. Gintexin A is Asn-49 type PLA2 isomer that has no enzymatic activity and neurotoxicity. Gentexin is a heterodimer comprising gintexin A and gentexin B. It is estimated that, after the venom enter the blood system GintexinA acts as a "director" to guide the neurotoxin heterodimer to bind to the membrane receptor at the phrenic nerve NMJ synapse, and then Gintexin B exerts neurotoxicity (blocks the diaphragm NMJ synaptic transmission and stops breathing). |
Alternative Name | Gintexin A; PLA2; phospholipase 2; GA-3C12 |
UniProt | A0A096XPP4 |
Immunogen | The original antibody was prepared by immunizing BALB/c mice with attenuated Gintexin antigen purified from the crude venom of Gloydius intermedius. |
Application Notes | This antibody was capable of recognizing attenuated gintexin A of Gloydius intermedius and a fusion protein containing gintexin A in a western blot assay. The binding affinity of this antibody for gintexin A was determined using ELISA. 100 μg this antibody can neutralize at least 2×LD50 of Agkistrodon venom. In vivo, the neutralizing activity of this antibody is reported to be about 21.1 LD50/mg. The sensitivity of HRP-N20 antibody for immunospot detection of Agkistrodon intermedius venom is 12.5 ng/mL. |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
