Anti-Giantin mAbs

Cat. No.

MABL-1694

Name

Anti-Giantin mAbs

Clone No.

AFD- TA10

From

Recombinant Antibody

Isotype

Engineer antibody

Application

in vivo, WB, IF

Species Reactivity

Rat, Human

Basic Information

Specificity

This antibody is specific for a 79 amino   acid long region between amino acids 1462 to 1540 of the full human sequence   (corresponding to amino acids 1399 to 1478 in the rat homologue) in Giantin,   a Golgi matrix protein.

Alternative Name

Golgin subfamily B member 1; 372 kDa Golgi complex-associated protein; GCP372; Macrogolgin

UniProt

Immunogen

The original antibody was generated via phage   display from the Griffin.1 library by screening against Rat liver Golgi (RLG)   complexes derived from rat liver.

Application Notes

The original format of this antibody (scFv)   was successfully used in a Western blot and for in vitro immunofluorescence   straining of Giantin in HeLa cells. siRNA knock-down of Giantin reduced TA10 staining, confirming its specificity. Furthermore, an in vivo expressed TA10-GFP construct successfully stained HeLa cells (Nizak et al., 2003; PMID: 14617357). The   scFv-Fc fusion version of this antibody — hTA10 — detected Giantin when cells   were fixed by paraformaldehyde (Moutel et al., 2009; PMID： 19245715). Precise   characterization of the TA10 target was achieved; a region of 79 amino acids,   representing less than 2.5% of the entire protein, was determined as its   binding site (Vielemeyer et al., 2010; PMID：20727208).

Antibody First   Published

Nizak et al. Recombinant antibodies against subcellular fractions used to track endogenous Golgi protein dynamics in vivo Traffic. 2003 Nov;4(11): 739-53. doi: 10.1034/j.1600- 0854.2003.00132.x PMID：14617357

Note on publication

The original publication demonstrates the   use of recombinant antibodies selected through phage display against   subcellular fractions.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.