Key features and details | |
Cat. No. | MABL-1724 |
Name | Anti-Glypican 3 mAbs |
Clone No. | AFD- 4G7 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA, FC, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody binds an epitope spanning the furin cleavage site at amino acids R358/S359 of human GPC3. Glypican-3 is an oncofetal antigen that belongs to the glypican family of glycosyl- phosphatidylinositol-anchored heparin sulfate proteoglycans. It negatively regulates the hedgehog signaling pathway and positively regulates the canonical Wnt signaling pathway. Multiple studies have revealed that GPC-3 is overexpressed in hepatocellular carcinoma (HCC). |
Alternative Name | GPC3; Glypican-3; GPC-3; GTR2-2; MXR7; OCI5; DGSX; Intestinal protein OCI-5 |
UniProt | P51654 |
Immunogen | The original antibody was generated by immunizing BALB/c mice with a recombinant human GPC3 extracellular domain (ECD, amino acids of 1-547) fused to a C-terminal Flag (RADYKDDDDK). |
Application Notes | This antibody can bind human glypican 3 expressed on the surface of 293S cells, HepG2 X1 cells and JHH7 cells in a flow cytometric assay. The binding characterization of this antibody to huGPC3, cynoGPC3, and HepG2 cells was done using ELISA and immunohistochemistry (US9926377). |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
