Key features and details | |
Cat. No. | MABL-1726 |
Name | Anti-Glypican 3 mAbs |
Clone No. | AFD- AB13E3 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | functional assay, ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody binds human glypican 3 (GPC3).Glypican-3 is an oncofetal antigen that belongs to the glypican family of glycosyl-phosphatidylinositol-anchored heparin sulfate proteoglycans. It negatively regulates the hedgehog signaling pathway and positively regulates the canonical Wnt signaling pathway. Multiple studies have revealed that GPC-3 is overexpressed in hepatocellular carcinoma (HCC). |
Alternative Name | GPC3; Glypican-3; GPC-3; GTR2-2; MXR7; OCI5; DGSX; Intestinal protein OCI-5; GPC3#4; DWX-1 |
UniProt | P51654 |
Immunogen | The parental mouse antibody GPC3#4 was generated by immunizing BALB/c mice with 50μg of purified human GPC-3 antigen emulsified in Freund's complete adjuvant. This original antibody was generated be grafting CDRs of the parental mouse antibody onto human framework region. |
Application Notes | The binding specificity of this antibody for human glypican 3 was determined using ELISA. The original IgG1 version of this antibody binds human GPC3 with an affinity of Kd = 2.84 pM. This antibody can bind GPC3 expressed on the surface of PLC/PRF/5 and Huh7 cells in an in vitro binding assay. This antibody also positively shows ADCC activity on PLC/PRF/5 cells expressing glypican 3 (CN109988240). |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
