Anti-GM1-bound Aβ mAbs

Cat. No.

MABL-1727

Name

Anti-GM1-bound Aβ mAbs

Clone No.

AFD-4396

From

Recombinant Antibody

Isotype

Engineer antibody

Application

IM, ThT fluorescence assay, WB, EM

Species Reactivity

Human

Basic Information

Specificity

This antibody is specific for human GM1-Aβ.

Alternative Name

Gaβ; GM1 ganglioside-bound amyloid   β-protein; 4396C

UniProt

Immunogen

The original antibody was generated by immunizing mice with purified human brain-derived GM1-Aβ.

Application Notes

The original mouse IgM antibody was   developed and tested to specifically recognize Aβ bound to GM1 (GM1-Aβ). A   subsequent version, 4396C, an IgG2a subtype chimeric antibody, was generated   and evaluated. The chimeric antibody retained its specificity for GM1-Aβ, as   demonstrated by electron microscopy (EM), where it specifically labeled   GM1-containing liposomes incubated with Aβ; Western blotting (WB), which   confirmed the antibody’s specificity for GM1-Aβ; a Thioflavin T (ThT) assay,   which showed that the antibody inhibited amyloid fibril formation in a   dose-dependent manner when incubated with GM1-containing liposomes and Aβ;   and immunoelectron microscopy (IM), where it effectively bound to newly   formed amyloid fibrils in the presence of synthetic Aβ and GM1-containing   liposomes (US7339035B2). 

Antibody First   Published

Note on publication

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.