Key features and details | |
Cat. No. | MABL-1758 |
Name | Anti-H3K27me3 mAbs |
Clone No. | AFD- 2E12LI |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | functional assay, immunofluorescence |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is capable of recognizing histone H3 proteins with a trimethylation at Lys27. It specifically recognizes and binds trimethyl lysine and amino acids surrounding it comprising KQLATKAAR(trimethyl K)SAPATGGVKC. Methylation is a type of post-translational modification (PTM) which is caused by covalent addition of methyl group to proteins, DNA or other molecules. PTMs have fundamental roles in chromatin structure and function. The PTMs that have taken place in histone proteins can affect gene expression by altering chromatin structure. Histone modifications have also been known as epigenetic markers. |
Alternative Name | Trimethylated Histone H3 Lys27; trimethyl-Histone H3 (Lys27); histone H3; H3K27; Trimethylated histone H3; Histone H3 trimethylated at Lysine 27; nucleosome; H3K27ME3; post translational modification; histone post-translational modification; 2E12 mutant M86L/M158I; 2E12 |
UniProt | |
Immunogen | The original antibody was generated by immunizing BALB/c mice with a 20-mer peptide (KQLATKAAR (trimethyl K) SAPATGGVKC) containing histone H3K27 trimethylated lysine and conjugated with KLH. |
Application Notes | This antibody was used for the development of a H3K27me3‐specific intracellular antibody or H3K27me3‐mintbody, which was a fusion protein comprising variable domain regions of 2E12LI antibody fused to a fluorescent protein and expressed in mouse teratocarcinoma cell line MC12. This mintbody was capable of tracking H3K27me3 in living cells. Mouse MC12 cells, which stably express H3K27me3‐mintbody (sfGFP) was used to localize H3K27me3‐enriched chromatin in living cells. The binding specificity of bacterially expressed and purified 2E12LI‐sfGFP in vitro was evaluated using a modified histone peptide array. (PMID: 33605056). |
Antibody First Published | Jian Xu et al. H4K20me1 and H3K27me3 are concurrently loaded onto the inactive X chromosome but dispensable for inducing gene silencing. EMBO Rep. 2021 Mar 3; 22(3): e51989. PMID:33605056 |
Note on publication | Describes the generation of an H3K27me3‐specific intracellular antibody for localization of histone PTMs. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
