Key features and details | |
Cat. No. | MABL-1759 |
Name | Anti-H3K27me3 mAbs |
Clone No. | AFD- BT164 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ICC, IP, WB, ELISA, IF, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | The epitope of BT164 was mapped in the N-terminal tail of histone H3 (27-KSAPAT-32) and included the apoptosis-induced trimethylation of K27. |
Alternative Name | trimethyl-Histone H3 (Lys27); histone H3; H3K27; Trimethylated histone H3; Histone H3 trimethylated at Lysine 27; nucleosome; H3K27ME3; post translational modification; histone post-translational modification |
UniProt | |
Immunogen | BT164 is a mice-derrived lupus antibody that was established by selection on apoptotic chromatin. |
Application Notes | BT164 recognizes an apoptosis-induced trimethylation of K27 on N-terminal tail of histone H3 which is a target for autoantibodies in patients and mice with SLE (systemic lupus erythematosus) (van Bavel et al., 2011; pmid: 20699234). Consequently, this antibody is recommended for the detection and studying of SLE. ELISA studies demonstrated that BT164 exhibits preferential activity with the apoptotic nucleosomes in comparison with the control nucleosomes (van Bavel et al., 2011; pmid: 20699234). It can be used for the detection of apoptosis in general. Furthermore, this antibody was demonstrated to label cells with methylated histone-3 on K27 via immunofluorescence and it also immunoprecipitated chromatin with such a methylation profile, further demonstrating its ability to specifically recognize markers characteristic of SLE (van Bavel et al., 2011; pmid: 20699234). |
Antibody First Published | van Bavel et al. Apoptosis-induced histone H3 methylation is targeted by autoantibodies in systemic lupus erythematosus. Ann Rheum Dis. 2011 Jan;70(1):201-7. doi: 10.1136/ard.2010.129320. Epub 2010 Aug 10. PMID:20699234 |
Note on publication | This article describes the generation and characterization of the antibody BT164. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
