Key features and details | |
Cat. No. | MABL-1761 |
Name | Anti-H3K4me3 mAbs |
Clone No. | AFD- 304M3-B |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | CHIP, ChIP-seq, IP, IP-MS, WB, ELISA, IF |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is highly specific for Histone H3 trimethylated at lysine 4. It has been tested on human histone H3 (K562 cells), but owing to the high conservation of histones, is expected to cross-react with many other species. |
Alternative Name | trimethyl-Histone H3 (Lys4); histone H3; H3K4; Trimethylated Histone H3; Trimethylated Lys; Trimethylated Lys4; Histone H3 trimethylated at Lysine 4; post translational modification; histone post-translational modification |
UniProt | B2R4P9 |
Immunogen | The biotinylated peptide from histone H3 ARTKQTARKSTG-GYCD. The last four amino acids GYCD were added for biotinylation purposes. |
Application Notes | This antibody has been used in IP-studies and works in immunofluorescence and Western Blot. In an long-neck scFv-Fc-fusion format been shown to exhibit a high degree of specificity owing to an antigen-clasping binding mechanism (see Hattori et al. 2016). |
Antibody First Published | Hattori et al. Antigen clasping by two antigen-binding sites of an exceptionally specific antibody for histone methylation. Proc Natl Acad Sci U S A. 2016 Feb 23;113(8):2092- 7 PMID:26862167 |
Note on publication | Describes the characterization of the specificty of this antibody through antigen- clasping. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
