Key features and details | |
Cat. No. | MABL-1768 |
Name | Anti-HA mAbs |
Clone No. | AFD- 5J8 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | crystallization, in vivo, inhibition, neutralization, ELISA |
Species Reactivity | H1N1 Influenza A virus |
Basic Information | |
Specificity | This antibody is specific for the H1N1 influenza virus hemagglutinin protein. It recognizses a conserved non-linear epitope (residues 133, 137 and 222) on the globular head of H1N1 HA between the receptor-binding pocket and the Ca2 antigenic site. The antibody inhibits all tested H1N1 influenza strains from 1918 to 1977 (A/South Carolina/1/1918, A/swine/Iowa/15/1930, A/Weiss/1943, A/L3/1947, A/New Jersey/11/1976, A/USSR/92/1977) and the pandemic 2009 virus but not the seasonal H1N1 strains from 1999 or 2007. |
Alternative Name | Hemagglutinin |
UniProt | G8XMJ2 |
Immunogen | The original antibody was isolated from the peripheral blood mononuclear cells of a healthy 47-year-old human subject. |
Application Notes | The crystal structure of the Fab fragment in complex with a bacterially expressed and refolded globular head domain from the hemagglutinin was determined (Hong et al., 2013; PMID: 24027321). The antibody was inhibitory in a hemagglutination inhibition activity against H1N1pdm2009-like virus. Neutralization capacity of the antibody was prooved by an MN-assay. The specificity of the antibody to the monomeric head as to the full HA-trimer was confirmed by ELISA (Aartse et al., 2021; PMID: 34358138). |
Antibody First Published | Krause et al. A broadly neutralizing human monoclonal antibody that recognizes a conserved, novel epitope on the globular head of the influenza H1N1 virus hemagglutinin J Virol. 2011 Oct;85(20):10905-8. PMID:21849447 |
Note on publication | The paper describes the generation and characterization of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
