Key features and details | |
Cat. No. | MABL-1773 |
Name | Anti-Haemoglobin mAbs |
Clone No. | AFD- 2A1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, ELISA |
Species Reactivity | Mouse |
Basic Information | |
Specificity | 2A1 exhibited polyreactive recognition of peptide 100-119 from the alpha subunit and peptide 100-119 frmo the beta subunit. Moreover, 2A1 bound Hb and the Hb-haptoglobin complex with equal efficiency, implying different binding sites on Hb for haptoglobin and 2A1. 2A1 was also found to distinguish between Hb and metHb. |
Alternative Name | Hemoglobin; Hb |
UniProt | P01942 |
Immunogen | Murine hybridomas were generated using spleen cells from aging (8–10 month old) NZB/W F1 animals. Hybridomas secreting antibodies reactive to murine hemoglobin were subcloned by limiting dilution and antibody isotypes determined using commercial reagents. Eight-week-old NZB/W F1 were immunized subcutaneously with 50 mg mHb antigen emulsified in Complete Fruend’s Adjuvant. |
Application Notes | 2A1 has been used to bind to mHb in Western Blots and has been found to increase the secretion of TNF-alpha from endothelial cells when co-incubated with Hb, suggesting that 2A1 induces pro-inflammatory events (Bhatnagar 2011). |
Antibody First Published | Bhatnagar et al. Serum and organ-associated anti-hemoglobin humoralautoreactivity: Association with anti-Sm responses and inflammation Eur. J. Immunol. 2011. 41: 537–548 PMID:21268022 |
Note on publication | Describes the presence of free hemoglobin in systemic lupus erythematosus and the investigation of anti-Hemoglobin autoimmune reactivity in patients. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
