Key features and details | |
Cat. No. | MABL-1774 |
Name | Anti-Hairpin DNA mAbs |
Clone No. | AFD- DNA-1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | crystallography, IP, ITC, ELISA |
Species Reactivity | Species independent |
Basic Information | |
Specificity | This antibody is specific for dT5, with high specificity to sequences greater than 5 nucleotides. It primarily recognizes the thymine DNA base, but it also binds to oligo(dT)15 nucleotides or greater and oligo(dT) of length three to five nucleotides. The antibody also recognizes the abbreviated hairpin sequence LIG5-14. |
Alternative Name | dT5; Oligo(dT); Oligo (dT); poly(dT); poly (dT) |
UniProt | |
Immunogen | The original format of this antibody was isolated from a phage display derived from MRL/lpr mice (autoimmune mice). The library was panned against single-stranded calf thymus DNA. |
Application Notes | The original format of this antibody (Fab) was generated and characterized. It was shown to bind DNA in an IP assay. and its binding affinity was determined to be (4.4±2.4) × 10-8 M (Calcuttet al., 1993; PMID: 7506692). Fluorescence titration of the antibody with dT5 was performed, and a Kd of~27 µM was measured (Komissarov et al., 1996; PMID: 8647821). The crystal structure of the Fab fragment in complex with dT5 was determined (Tanner et al., 2001; PMID: 11733999). Isothermal titration calorimetry experiments were used to measure the apparent affinity of the antibody for dT5. The apparent association constant was KA = 6.0 × 104 M-1. Furthermore, the binding of dT5 to the antibody was exothermic, with an apparent ΔHcal under solution conditions of -16.8 kcal/mol (Schuermann et al., 2004; PMID: 15340914). |
Antibody First Published | Calcutt et al. Isolation and characterization of nucleic acid-binding antibody fragments from autoimmune mice-derived bacteriophage display libraries Gene. 1993 Dec 27;137(1):77-83. PMID:7506692 |
Note on publication | The original publication explores the isolation and characterization of nucleic acid- binding antibody fragments from autoimmune mice-derived bacteriophage display libraries. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
