Key features and details | |
Cat. No. | MABL-1775 |
Name | Anti-hapten 5 mAbs |
Clone No. | AFD-17E 8 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Catalytic assays, crystallization, ELISA |
Species Reactivity | Species independent |
Basic Information | |
Specificity | This antibody is specific for norleucine and methionine phenyl esters enantiomers that possess the natural S configuration (L) at the α-carbon. |
Alternative Name | Noracine phosphonate; Norleucine phenyl phosphonate analog 1; Diphenyl [1-(1-N-Succinylamino)pentyl]phosphonate; Phosphonate transition state analog (hapten) |
UniProt | |
Immunogen | The original antibody was generated by immunizing Balb/c mice with the KLH conjugate of hapten 5 (norleucine phenyl phosphonate analog 1; Diphenyl [1-(1-N-Succinylamino)pentyl]phosphonate). |
Application Notes | This antibody efficiently catalyzes the hydrolysis of N-formyl norleucine phenyl ester and N-formyl methionine phenyl ester, with a preference for N-formyl esters over N-acetyl esters while demonstrating a superior catalytic activity for ester hydrolysis compared to other catalytic antibodies. The catalytic efficiency is influenced by the structural dissimilarity of the substrate to the antigen at specific positions. Notably, the original format of this antibody (mouse IgG2b) exhibits a higher affinity for ester substrates with norleucine side chains than methionine side chains. This original antibody's catalytic activity showed a bell-shaped pH dependence, reaching its maximum at pH 9.5. This pH optimum is attributed to the specific ionizable groups in the antibody's active site, contributing to efficient catalysis. |
Antibody First Published | Guo et al. Kinetic and Mechanistic Characterization of an Efficient Hydrolytic Antibody: Evidence for the Formation of an Acyl Intermediate Journal of the American Chemical Society,116(14), 6062-6069. https://doi.org/10.1021/ja00093a002 |
Note on publication | The original publication presents the generation and mechanistic characterization of a highly active catalytic antibody that exhibits hydrolytic activity towards norleucine and methionine esters. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
