Anti-HB-EGF mAbs

Cat. No.

MABL-1776

Name

Anti-HB-EGF mAbs

Clone No.

AFD- KM3566

From

Recombinant Antibody

Isotype

Engineer antibody

Application

inhibition, neutralization, WB, ELISA, FC

Species Reactivity

Human

Basic Information

Specificity

The antibody is specific for HB-EGF.

Alternative Name

HBEGF; Proheparin-binding EGF-like growth factor; Heparin-binding EGF- like growth factor; Diphtheria toxin receptor; DT-R

UniProt

Q99075

Immunogen

The original antibody was generated by   immunizing mice with human HB-EGF.

Application Notes

The specificity of the original format of   the antibody was confirmed by ELISA analysis. The antibody detected the   recombinant secretory human HB-EGF by western blot analysis. The HB-EGF- EGFR   binding inhibitory activity of the original antibody was examined using   32D/EGFR cell and biotin- labeled HB-EGF. The original antibody   concentration-dependently inhibited binding of biotinylated HB-EGF to EGFR.   Further, it could also inhibit binding of HB-EGF with EGFR. The neutralizing   activity of the original antibody was examined by a cell growth inhibition   assay which used an HB-EGF-dependent cell. The original antibody showed   HB-EGF-dependent cell growth inhibition activity for the cell line 32D/EGFR. The original antibody bound to the membrane type and cell membrane-bound   HB-EGF of cancer cell lines (human gastric cancer cell line MKN-28, human  ovarian cancer cell line ES-2, human breast cancer cell line MDA-MB-231).

Antibody First   Published

Note on publication

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.