Anti-HCEC mAbs

Cat. No.

MABL-1780

Name

Anti-HCEC mAbs

Clone No.

AFD- FC44

From

Recombinant Antibody

Isotype

Engineer antibody

Application

in vitro, in vivo, ELISA

Species Reactivity

Rat, Human

Basic Information

Specificity

The antibody is specific for HCEC.

Alternative Name

Human cerebromicrovascular endothelial cells

UniProt

Immunogen

The original antibody was isolated from a naive   phage displayed library of llama VHH. The library was panned against HCEC target cells.

Application Notes

The specificity of the original format of   the antibody was confirmed by ELISA analysis (US8715659B2; Muruganandam et al., 2002; PMID: 11772942). The original format of the antibody bound to the   vesicular and the membrane fractions of HCEC, and it was taken up by living   HCEC, as shown by fluorescence microscopy. The antibody could transmigrate   across an in vitro human blood-brain barrier (BBB) model. The antibody was also also detected in the brain after i.v. injection into mice (Muruganandam   et al., 2002; PMID: 11772942). The antibody was able to transport across rat   BBB, as demonstrated by in vitro and in vivo studies (Xiao et al., 2013;   PMID: 23840214).

Antibody First   Published

Muruganandam et al. Selection of   phage-displayed llama single-domain antibodies that transmigrate across human   blood-brain barrier endothelium FASEB J. 2002 Feb;16(2):240-2. doi:   10.1096/fj.01-0343fje. Epub 2001 Dec 28. PMID:11772942

Note on publication

The original paper describes the generation   and characterization of the antibody.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.