Key features and details | |
Cat. No. | MABL-1808 |
Name | Anti-Hepcidin-25 mAbs |
Clone No. | AFD- 23F11 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for amino acids 'DTHFPICIFCCGCCHRSKCGMCCKT' of human hepcidin- 25 and does not cross react with murine hepcidin-25. Hepcidin is a an iron-regulating peptide hormone made in the liver. Hepcidin is a cationic, cysteine-rich and tightly folded peptide stabilized by 4 disulfide bonds that plays a major role in innate immunity and iron homeostasis. It controls the delivery of iron to blood plasma from intestinal cells absorbing iron, from erythrocyte-recycling macrophages, and from iron- storing hepatocytes. Hepcidin acts by binding to and inactivating the sole cellular iron exporter, ferroprotein, which delivers iron to plasma from all iron-transporting cells. |
Alternative Name | HAMP; hHepc; HEPC; LEAP1; LEAP-1; Hepcidin; Liver-expressed antimicrobial peptide 1 |
UniProt | P81172 |
Immunogen | The original antibody was generated by immunizing Xenomouse™ IgG2κλ and IgG4κλ with KLH-conjugated human hepcidin peptide 'DTHFPICIFCCGCCHRSKCGMCCKT'. |
Application Notes | The original IgG antibody binds human hepcidin-25 with a binding affinity of Kd= 70 pM (PMID： 19553669). This antibody can be used in combination with another anti-hepcidin antibody 19D12 for detection of human hepcidin in human serum using a sandwich ELISA. 19D12 was used as a capture antibody and 23F11 was used for detection. The limit of sensitivity for the assay was 30 pg/mL (PMID： 23945155). |
Antibody First Published | Cooke et al. A fully human anti-hepcidin antibody modulates iron metabolism in both mice and nonhuman primates.Blood. 2013 Oct 24;122(17)：3054-61. PMID：23945155 |
Note on publication | Describes the development of a fully human antibody for use as a potential therapeutic to treat anemia of inflammation (AI) in patients with kidney disease or cancer. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
