Anti-Hepcidin-25 mAbs

Cat. No.

MABL-1809

Name

Anti-Hepcidin-25 mAbs

Clone No.

AFD- 4C11

From

Recombinant Antibody

Isotype

Engineer antibody

Application

ELISA

Species Reactivity

Rat, Human, Mouse

Basic Information

Specificity

This antibody binds human hepcidin-25 in the   N-terminus region. This antibody also cross reacts with mouse and rat   hepcidin-2, to a much lesser degree, human hepcidin-22 but not human   hepcidin- 20. Hepcidin is a an iron-regulating peptide hormone made in the   liver. Hepcidin is a cationic, cysteine-rich and tightly folded peptide   stabilized by 4 disulfide bonds that plays a major role in innate immunity   and iron homeostasis. It controls the delivery of iron to blood plasma from   intestinal cells absorbing iron, from erythrocyte-recycling macrophages, and   from iron-storing hepatocytes. Hepcidin acts by binding to and inactivating   the sole cellular iron exporter, ferroprotein, which delivers iron to plasma   from all iron- transporting cells.

Alternative Name

HAMP; hHepc; HEPC; LEAP1; LEAP-1; Hepcidin; Liver-expressed antimicrobial peptide 1; hepcidin-22

UniProt

P81172

Immunogen

The original antibody was generated by   immunizing mice with immunized with a N-terminal hepcidin peptide 'DTHFPIC' conjugated with a five amino acid peptide linker (i.e., GPGPG) to an OVA peptide (amino acids 323-336) sequence, i.e., full-length immunogen   DTHFPICGPGPGISQAVHAAHAEINE.

Application Notes

This antibody can be used for the detection of human, rat or mouse hepcidin-25 using ELISA.This antibody can also detecthuman hepcidin-22 with a binding affinity of Kd= 209nM (US8609817).

Antibody First   Published

Note on publication

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.