Anti-HGF mAbs

Cat. No.

MABL-1820

Name

Anti-HGF mAbs

Clone No.

AFD- 1A3

From

Recombinant Antibody

Isotype

Engineer antibody

Application

in vivo, SPR, WB, ELISA

Species Reactivity

Human

Basic Information

Specificity

The antibody is specific for human HGF.

Alternative Name

SF; Hepatocyte growth factor;   Hepatopoietin-A; Scatter factor

UniProt

P14210

Immunogen

The original antibody was generated by immunizing   Balb/c mice with recombinant human HGF.

Application Notes

The specificity of the original format of   the antibody was confirmed by ELISA analysis (IC50 = 5.65 nM). The binding   affinity of the original format of the antibody to human HGF was measured by   surface plasmon resonance (Kd= 30.1 pM). The antibody could bind to  non-reduced and reduced rhHGF by western blot analysis. The antibody could   efficiently neutralize HGF binding to c-Met. The antibody inhibited HGF   induced proliferation in 4MBr-5 cells. The ability of the original format of   the antibody to inhibit tumor growth was tested in an U87MG xenograft model.   The percent of inhibition of the antibody was measured to be 73%. The ability of the original format of the antibody to inhibit tumor growth was tested in an U118 xenograft model. The percent of inhibition of the antibody was   measured to be 57% (US7659378B2).

Antibody First   Published

Note on publication

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.