Anti-hINSR mAbs

Cat. No.

MABL-1827

Name

Anti-hINSR mAbs

Clone No.

AFD- IR 47-46

From

Recombinant Antibody

Isotype

Engineer antibody

Application

Blocking, IB, IP

Species Reactivity

Bovine, Human

Basic Information

Specificity

This antibody reacts with human INSR,  recognising an epitope on the alpha subunit (amino acids 485-592). It does   not cross-react with rat or mouse insulin receptors, and does cross-react   with bovine and rabbit receptors.

Alternative Name

CD220; human insulin receptor; insulin   receptor; HHF5; IR; 47 46

UniProt

P06213

Immunogen

This antibody was raised by immunising BALB/c mice with IM-9 lymphocytes, and boosting with purified human placental   insulin receptor prior to fusion.

Application Notes

This antibody has been used in   immunoprecipitation assays with radiolabelled human insulin receptor (hINSR)   (Soos et al, 1986). It has also been used in immunoblot analysis with   purified hINSR (Soos et al, 1986), revealing that it binds to the receptor’s   alpha subunit. This antibody has been shown to cause >80% inhibition of   insulin binding to IM-9 cells (Soos et al, 1986), and to block receptor   activation.

Antibody First   Published

Soos et al. Monoclonal antibodies reacting with   multiple epitopes on the human insulin receptor. Biochem J. 1986 Apr 1;  235(1)： 199–208. PMID：2427071

Note on publication

Describes the original generation of this   antibody, and the characterisation of its specificity.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.