Anti-hINSR mAbs

Cat. No.

MABL-1828

Name

Anti-hINSR mAbs

Clone No.

AFD- IR 83-16

From

Recombinant Antibody

Isotype

Engineer antibody

Application

IP, stimulatory

Species Reactivity

Bovine, Rabbit, Human

Basic Information

Specificity

This antibody reacts with human INSR,   recognising an epitope on the alpha subunit (amino acids 191-290). It does   not cross-react with rat insulin receptors, and does cross-react with rabbit,   bovine, porcine and ovine receptors.

Alternative Name

CD220; human insulin receptor; insulin   receptor; HHF5; IR; 83 16

UniProt

P06213

Immunogen

This antibody was raised by immunising   BALB/c mice with IM-9 lymphocytes, and boosting with purified human placental   insulin receptor prior to fusion.

Application Notes

This antibody has been used in   immunoprecipitation assays, with radiolabelled human insulin receptor (hINSR)   and hINSR-radiolabelled insulin complexes (Soos et al, 1986). This antibody   has been shown to enhance insulin binding to IM-9 cells (Soos et al, 1986).

Antibody First   Published

Soos et al. Monoclonal antibodies reacting   with multiple epitopes on the human insulin receptor. Biochem J. 1986 Apr 1;  235(1)： 199–208. PMID：2427071

Note on publication

Describes the original generation of this   antibody, and the characterisation of its specificity.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.