Anti-hINSR mAbs

Cat. No.

MABL-1829

Name

Anti-hINSR mAbs

Clone No.

AFD- IR 83-22

From

Recombinant Antibody

Isotype

Engineer antibody

Application

IB, inhibitory, IP

Species Reactivity

Human

Basic Information

Specificity

This antibody is specific for human INSR,   reacting with an epitope in the alpha subunit (amino acids 485-592). It does   not cross-react with rabbit, rat or mouse insulin receptors, and displays   very weak cross-reactivity with bovine, porcine and ovine receptors.

Alternative Name

CD220; human insulin receptor; insulin   receptor; HHF5; IR; 83 22

UniProt

P06213

Immunogen

This antibody was raised by immunising   BALB/c mice with IM-9 lymphocytes, and boosting with purified human placental   insulin receptor prior to fusion.

Application Notes

This antibody has been used in immunoprecipitation   assays, with both radiolabelled hINSR and hINSR-radiolabelled insulin   complexes (Soos et al, 1986). It has also been used in immunoblot analysis   with purified hINSR (Soos et al, 1986), revealing that it binds to the   receptor’s alpha subunit. This antibody has been shown to cause >80%   inhibition of insulin binding to IM-9 cells (Soos et al, 1986), and behaves   as a surrogate agonist.

Antibody First   Published

Soos et al. Monoclonal antibodies reacting   with multiple epitopes on the human insulin receptor. Biochem J. 1986 Apr 1;  235(1): 199–208. PMID: 2427071

Note on publication

Describes the original generation of this   antibody, and the characterisation of its specificity.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.