Key features and details | |
Cat. No. | MABL-1839 |
Name | Anti-HIV p24 antibody; HIV gp120 antibody mAbs |
Clone No. | AFD- 1F7 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | in vivo, WB, ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | The anti-idiotypic antibody recognizes antibodies that react to different components of the HIV virus, such as gp120, p24. The antibody does not react with antibodies derived from SLE and B cell lymphoma. |
Alternative Name | ATCC No. HB 11286; anti-idiotypic antibody |
UniProt | |
Immunogen | The original antibody was generated by immunizing BALB/c mice with pooled human polyclonal HIVIG. |
Application Notes | The HIV-1 antigen capture ELISA was used to test the binding of human monoclonal anti-HIV-1 to the antibody. The antibody bound to anti-p24 antibodies and anti-gp120 antibodies. Western blot analysis confirmed that the antibody reacts with human antibodies of different HIV-1 antigen specificities (Wang et al., 1992; PMID: 1378015). SHIV-infected macaques were immunized with the antibody. Significant increases in breadth and potency of HIV-1 neutralizing antibody titers to one or more virus strains were detected in all three of the 1F7 immunized macaques (US6057421A). |
Antibody First Published | Müller et al. Generation and specificity of monoclonal anti-idiotypic antibodies against human HIV-specific antibodies. I. Cross-reacting idiotopes are expressed in subpopulations of HIV-infected individuals. J Immunol. 1991 Aug 1;147(3):933-41. PMID:1861082 |
Note on publication | The paper describes the generation and characterization of the anti-idiotypic antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
