Key features and details | |
Cat. No. | MABL-1840 |
Name | Anti-HIV-1 protease mAbs |
Clone No. | AFD- F11.2.32 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | neutralize, ELISA |
Species Reactivity | HIV-1 |
Basic Information | |
Specificity | F11.2.32 Fab binds to HIV-1 PR with a dissociation constant of 5 nM (determined by SPR at pH5.2) at an epitope consisting of MSLPGRWKPKM (aa 36-46). HIV-1 PR is a homodimeric apartate protease that specifically cleaves the viral Gag and Gag/Pol polyprotein precursor. A correlation has been observed between the activity of the protease and the degree of infectivity, and so this protein is a major antiviral target. |
Alternative Name | HIV-1 PR; HIV1 PR; HIV1 protease |
UniProt | O90777 |
Immunogen | F11.2.32 was prepared by generating a hybridoma cell line in which myeloma cells were fused with splenocytes derived from BALB/c mice immunized with HIV-1 PR in the presence of Freund's adjuvant. F11.2.32 was selected for its ability to bind to HIV-1 PR by ELISA. |
Application Notes | The IgG and Fab variants of F11.2.32 are both able to strongly inhibit the catalytic activity of HIV-1 PR (IC50 ~35 nM). Inhibition occurs by distorting the native structure of the HIV-1 PR at the base of the flap region, which plays an important role in regulating access to the active site. This antibody is also able to detect HIV-1 PR by ELISA. |
Antibody First Published | Lescar et al. Preliminary crystallographic studies of an anti-HIV- 1 protease antibody that inhibits enzyme activity Protein Sci. 1996 May;5(5):966-8. PMID:8732768 |
Note on publication | Describes the generation of the mAb F11.2.32 and its crystallization in complex with peptide fragments of HIV-1 PR. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
