Key features and details | |
Cat. No. | MABL-1852 |
Name | Anti-HLA-A2 mAbs |
Clone No. | AFD- MBW1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | functional assay, ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | The antibody specifically binds human HLA-A2 and does not cross react with A28, Aw68, or Aw69 antigens. |
Alternative Name | HLA-A; Human leukocyte antigen A; HLA class I histocompatibility antigen; A-2 alpha chain; MHC class I antigen A-2 HLA-A; HLA class I histocompatibility antigen A-28 alpha chain; HLA class I histocompatibility antigen; A-28 alpha chain; MHC class I antigen A-28 |
UniProt | P04439 |
Immunogen | The original antibody was generated from the peripheral blood lymphocytes obtained from a multiparous volunteer 1 week postpartum were transformed in vitro by Epstein-Barr virus and electro fused with the heterohybridoma fusion partner, K6H6/B5. The screening for specific clone was done using micro lymphocytotoxicity assay. |
Application Notes | This antibody specifically reacts with human HLA-A2 and does not cross react with A28, Aw68, or Aw69 antigens (PMID: 2160447). The reactivity of this antibody to human HLA-A2 was determined using ELISA. The reactivity of this antibody towards HLA-A2 was abolished by substitutions in the a1 domain, specifically the substitutions of alanine for glutamic acid at position 58 (E58A) and either a valine or arginine substitution for glycine at position 62 (G62V or G62R). The reactivity of this antibody to surface bound HLA- A*0201 was determined using complement-dependent cytotoxicity (CDC) assay (PMID: 9862700). |
Antibody First Published | Wallace et al. Generation of a human hybridoma producing a pure anti-HLA- A2 monoclonal antibody. Hum Immunol. 1990 May;28(1):65-9. PMID:2160447 |
Note on publication | Describes the generation and characterization of this antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
