Key features and details | |
Cat. No. | MABL-1858 |
Name | Anti-HLA-DR mAbs |
Clone No. | AFD- 1D10 (Hu1D10; Apolizumab) |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, therapeutic, FC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody recognizes a variant epitope on the beta chain of HLA-DR. It can bind some but not all HLA-DR beta chains. The antigen that this antibody binds is expressed on lymphocytes, macrophages, and dendritic (mesenchymal) cells and on most B cell neoplasms. HLA-DR is an MHC class II cell surface receptor encoded by the human leukocyte antigen complex on chromosome 6 region 6p21.31. HLA-DR is also involved in several autoimmune conditions, disease susceptibility and disease resistance. |
Alternative Name | CD74; HLA class II histocompatibility antigen gamma chain; HLA-DR antigens-associated invariant chain; Ia antigen-associated invariant chain; Ii; p33 |
UniProt | P04233 |
Immunogen | The original mouse antibody 1D10 was generated by immunizing female BALB/c mice intraperitoneally with human large cell lymphoma HO-85. Humanized version of this antibody was generated by grafting CDRs of the parental mouse antibody onto human framework regions. |
Application Notes | This antibody is the humanized version of anti-HLA-DR mouse parental antibody called 1D10. This antibody is capable of recognizing a majority of B-cell malignancies. This antibody is capable of inducing complement-mediated cytotoxicity, antibody dependent cell cytotoxicity and direct apoptosis of B cells expressing HLA-DR. The original mouse version does not posses these characteristics. This antibody has similar affinity to human HLA-DR as compared to its parental mouse antibody (PMID: 11477560). This antibody was also used for the generation of a bispecific antibody in combination with anti-CD3e antibody M291 (Visilizumab) (US6129914). |
Antibody First Published | Kostelny et al. Humanization and characterization of the anti-HLA-DR antibody 1D10. Int J Cancer. 2001 Aug 15;93(4):556-65. PMID:11477560 |
Note on publication | This paper describes the generation and characterization of the humanized version of anti-HLA DR antibody 1D10. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
