Key features and details | |
Cat. No. | MABL-1881 |
Name | Anti-HPi2 mAbs |
Clone No. | AFD- HIC1-2B4 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | FC, IF, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody binds the HPi2 marker on the surface of human pancreatic enriched islet cells. |
Alternative Name | Human Pancreas Endocrine Islet Cell Marker 2; Human pancreatic marker; Endocrine pan-islet cell marker |
UniProt | |
Immunogen | The original antibody was generated by immunizing BALB/c mice with endocrine cells of the islets of Langerhans. |
Application Notes | This antibody binds distinct surface molecules expressed on endocrine cells and have been validated for a number of experimental methods including immunohistochemistry and live cell sorting by flow cytometry. This antibody can be used individually or in combination with others to isolate populations of α, β, exocrine, or duct cells from primary human pancreas by FACS and to characterize the detailed cell composition of human islet preparations (PMID: 19383399, 27641999). This antibody was used in combination with HIC3-2D12 to identify and isolate major subtypes of the human islet cells including α- and β-cells and the purity levels of the isolated cells was determined using immunofluorescence staining (PMID: 31780397). Humanized version of this antibody has also been reported (PMID: 29758224). |
Antibody First Published | Dorell et al. Isolation of major pancreatic cell types and long-term culture- initiating cells using novel human surface markers. Stem Cell Res. (2008); 1(3):183-94. PMID:19383399 |
Note on publication | Describes the development of a panel of antibodies for the isolation and study of major cell types of the human pancreas. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
